The University of Iowa in 2011 rejected a graduate application from James Holmes -- the alleged Aurora, Colorado, gunman -- with one official saying "Do NOT offer admissions under any circumstances," according to documents obtained by CNN.
A second university official agreed not to make the recommendation for Holmes' admittance.
Holmes, 24, is accused of opening fire at an Aurora theater last month during a midnight premiere of the latest Batman movie, "The Dark Knight Rises," killing 12 people and injuring 58 others.
He has been charged with murder and attempted murder and faces two weapons charges.
Holmes is expected to appear at a court hearing Thursday afternoon, which will address his contact with University of Colorado psychiatrist Lynne Fenton and a notebook he mailed to her before the shooting.
The suspected gunman was a doctoral candidate studying neuroscience at the University of Colorado's Anschutz campus in Aurora. But he was "denied access to the school after June 12, 2012, after he made threats to a professor," according to court documents.
Subsequently, Holmes "started the process to voluntarily withdraw from his graduate studies program."
The University of Colorado said this month that it hired a former U.S. attorney to conduct an independent review into how the school handled Holmes.
Holmes said earlier that he wanted to study "the primary source of all things, our own minds," according to a personal statement he submitted as part of a graduate studies application at the University of Illinois.
The application included the statement, professional references and test scores.
The documents were released by the university, where Holmes applied to the neuroscience department before later opting to attend the University of Colorado.
While the documents do little to answer questions about the alleged suspect's possible motive, they offer insight into Holmes as a student and his aspirations to study the human brain.
In the statement, Holmes wrote that he has long been "fascinated by the complexities of long lost thought seemingly arising out of nowhere into stream of awareness.
"These fascinations likely stemmed from my interest in puzzles and paradoxes as an adolescent and continued through my curiosity in academic research," he wrote in the statement, submitted in early 2011.
He titled his resume "aspiring scientist."
Holmes was described as taking "an active role in his education, and brings a great amount of intellectual and emotional maturity into the classroom," according to a letter of reference that appeared to be from one of his former professors.
Copyright 2012 Scripps Media, Inc. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed.
Did You Hear?
A teen who learned he didn't have much longer to live turned to writing music -- and his farewell song, "Clouds," became a YouTube sensation that has attracted more than 4 million views.
An Oklahoma man found his damaged truck and was able to start its engine, which put a smile on his face in the aftermath of a devastating tornado.
The Oscar Mayer Wienermobile will be visiting four Bashas’ stores leading up to Memorial Day weekend.
An interview with CNN's Wolf Blitzer and an Oklahoma mother became one of the most searched topics on the internet Wednesday.